5XPY
Structural basis of kindlin-mediated integrin recognition and activation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 145.069, 145.069, 59.352 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.408 - 2.099 |
R-factor | 0.1655 |
Rwork | 0.164 |
R-free | 0.19590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xpz |
RMSD bond length | 0.007 |
RMSD bond angle | 0.966 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.408 | 2.140 |
High resolution limit [Å] | 2.099 | 2.100 |
Rmerge | 0.151 | 0.761 |
Rpim | 0.043 | |
Number of reflections | 42158 | |
<I/σ(I)> | 19.9 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.1 M Tris pH 8.5, 4.0 M Ammonium acetate |