5XPX
Structure elucidation of truncated AMS3 lipase from an Antarctic Pseudomonas
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-08 |
Detector | RIGAKU |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 86.753, 94.693, 126.431 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.770 |
R-factor | 0.22463 |
Rwork | 0.222 |
R-free | 0.27710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fdm |
RMSD bond length | 0.013 |
RMSD bond angle | 1.689 |
Data scaling software | HKL-3000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.820 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 27263 | |
<I/σ(I)> | 9.4 | |
Completeness [%] | 99.7 | |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.1 M HEPES sodium pH 7.5, 0.8 M Potassium sodium tartrate tetrahydrate |