5XPW
Structure of amphioxus IgVJ-C2 molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98051 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 41.755, 41.755, 213.719 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.253 - 2.003 |
| R-factor | 0.2281 |
| Rwork | 0.226 |
| R-free | 0.26600 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.828 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.094 | 0.439 |
| Number of reflections | 15493 | |
| <I/σ(I)> | 24.8 | |
| Completeness [%] | 98.2 | 93.1 |
| Redundancy | 15.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M lithium sulfate monohydrate, 0.1M BIS-TRIS (pH 5.5), 25% w/v polyethylene glycol 3350 |
