5XPS
Crystal Structure of Transketolase in complex with erythrose-4-phosphate from Pichia Stipitis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-11 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.149, 185.246, 98.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 92.620 - 1.070 |
| R-factor | 0.11919 |
| Rwork | 0.119 |
| R-free | 0.13154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hyv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.515 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.620 | |
| High resolution limit [Å] | 1.070 | 1.070 |
| Rmerge | 0.058 | |
| Number of reflections | 403372 | |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES, 0.1M NaCl, PEG400 |
