5XPF
High-resolution X-ray structure of the T26H mutant of T4 lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.8000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 59.839, 59.839, 95.828 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.580 - 1.040 |
Rwork | 0.159 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qt8 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.029 |
Data scaling software | HKL-2000 |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.080 |
High resolution limit [Å] | 1.040 | 1.040 |
Rmerge | 0.041 | 0.361 |
Rmeas | 0.045 | 0.422 |
Rpim | 0.019 | 0.215 |
Number of reflections | 95774 | 9481 |
<I/σ(I)> | 51.6 | 4.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.4 | 3.7 |
CC(1/2) | 0.886 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium-potassium phosphate, Sodium chloride, 1,6-Hexanediol |