5XPF
High-resolution X-ray structure of the T26H mutant of T4 lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.839, 59.839, 95.828 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.580 - 1.040 |
| Rwork | 0.159 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qt8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.029 |
| Data scaling software | HKL-2000 |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.080 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.041 | 0.361 |
| Rmeas | 0.045 | 0.422 |
| Rpim | 0.019 | 0.215 |
| Number of reflections | 95774 | 9481 |
| <I/σ(I)> | 51.6 | 4.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.4 | 3.7 |
| CC(1/2) | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium-potassium phosphate, Sodium chloride, 1,6-Hexanediol |
