5XPA

Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 9'U10' on the target strand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298
Detector technology	CCD
Collection date	2012-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97892
Spacegroup name	P 43 21 2
Unit cell lengths	109.134, 109.134, 184.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.150 - 2.900
R-factor	0.218
Rwork	0.215
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kpy
RMSD bond length	0.006
RMSD bond angle	0.979
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.900
Number of reflections	25323
<I/σ(I)>	46.5
Completeness [%]	99.8
Redundancy	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	308	3.1 M NH4-acetate, 0. 1 M Bis-Tris propane, pH 7.3