5XP8
Crystal structure of T. thermophilus Argonaute protein complexed with a bulge 4A5 on the guide strand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 2014-12-08 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.1000 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 202.140, 202.140, 202.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.200 - 3.100 |
R-factor | 0.217 |
Rwork | 0.214 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kpy |
RMSD bond length | 0.022 |
RMSD bond angle | 1.633 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 25862 |
<I/σ(I)> | 9.7 |
Completeness [%] | 98.9 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 308 | 2.6 M Na-acetate, pH 7.0, 0.1M Tris-Cl, pH 7.4 and 0.2 M glycine |