5XP1
Structure of monomeric mutant of REI immunoglobulin light chain variable domain crystallized at pH 6
Replaces: 5B3CExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-05-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 4 |
Unit cell lengths | 130.760, 130.760, 92.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.961 - 2.880 |
R-factor | 0.2588 |
Rwork | 0.257 |
R-free | 0.29720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2q20 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.937 |
Data reduction software | iMOSFLM (V7.0.9) |
Data scaling software | SCALA (V3.3.20) |
Phasing software | MOLREP (V11.0.05) |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.961 | 2.930 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmerge | 0.080 | 0.519 |
Number of reflections | 35217 | 5153 |
<I/σ(I)> | 21.3 | 5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.7 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293.15 | 0.1M BIS-TRIS pH 6.0, 18% POLYETHYLENE GLYCOL 3350, 0.12M AMMONIUM SULFATE |