5XP0

Crystal structure of master biofilm regulator CsgD regulatory domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 41 21 2
Unit cell lengths	57.758, 57.758, 207.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.355 - 2.000
R-factor	0.209
Rwork	0.206
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.286
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.355
High resolution limit [Å]	2.000
Number of reflections	24586
<I/σ(I)>	15.7
Completeness [%]	99.1
Redundancy	24.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	(1) 1.6M magnesium sulfate heptahydrate, 0.1M MES, pH 6.5 (2) 1.5M ammonium sulfate, 0.1M BIS-TRIS propane pH 7.0