5XOM
Hydra Fam20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.997 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 80.221, 123.222, 144.394 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.806 - 2.200 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.21080 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.233 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 73294 |
| <I/σ(I)> | 2.9 |
| Completeness [%] | 99.7 |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 1.5 M (NH4)2SO4, 0.1 M BIS-TRIS propane, pH 7.0, and 3% dextran sulfate |
