5XOI
The structure of OsALKBH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-07 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.254, 67.633, 67.921 |
| Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
| Resolution | 39.943 - 1.800 |
| R-factor | 0.1818 |
| Rwork | 0.181 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3khb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.890 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.388 |
| Rpim | 0.059 | 0.246 |
| Number of reflections | 21158 | |
| <I/σ(I)> | 21.6 | 2.71 |
| Completeness [%] | 96.4 | 95.7 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | MPD(2-methyl-1,3-propanediol) , Tris base |
