5XOD
Crystal structure of human Smad2-Ski complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2015-11-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 110.840, 110.840, 110.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.250 - 1.851 |
| R-factor | 0.2328 |
| Rwork | 0.232 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mjs |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.585 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.250 | 45.250 | 1.890 |
| High resolution limit [Å] | 1.850 | 9.070 | 1.850 |
| Rmerge | 0.035 | 0.020 | 0.529 |
| Rmeas | 0.037 | 0.022 | 0.583 |
| Rpim | 0.012 | 0.007 | 0.242 |
| Total number of observations | 180013 | 1667 | 6579 |
| Number of reflections | 37448 | ||
| <I/σ(I)> | 28 | 92.7 | 2.9 |
| Completeness [%] | 99.8 | 99.5 | 97.7 |
| Redundancy | 9.3 | 8.8 | 5.7 |
| CC(1/2) | 1.000 | 0.999 | 0.442 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M sodium acetate tri-hydrate pH 4.6 and 1.85 M sodium formate |
