5XO2
Crystal structure of human paired immunoglobulin-like type 2 receptor alpha with synthesized glycopeptide II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.280, 63.329, 55.072 |
| Unit cell angles | 90.00, 110.08, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.201 |
| R-factor | 0.2286 |
| Rwork | 0.216 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wv0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 13393 | |
| <I/σ(I)> | 25 | 8.4 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES monohydrate pH 6.5, 1.6M Magnesium sulfate heptahydrate |
