5XNV

Crystal structure of YEATS2 YEATS bound to H3K27ac peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	I 4 2 2
Unit cell lengths	72.826, 72.826, 125.232
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.773 - 2.696
R-factor	0.2285
Rwork	0.224
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tmp
RMSD bond length	0.010
RMSD bond angle	1.268
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.800
High resolution limit [Å]	2.696
Number of reflections	4915
<I/σ(I)>	17.53
Completeness [%]	99.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	291	0.2 M lithium sulfate, 2.0 M ammonium sulfate, 0.1M CAPS, pH 10