5XNT

Structure of CYP106A2 from Bacillus sp. PAMC 23377

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 61 2 2
Unit cell lengths	77.658, 77.658, 282.216
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.680 - 2.700
R-factor	0.19406
Rwork	0.191
R-free	0.25674
RMSD bond length	0.013
RMSD bond angle	1.656
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	14704
<I/σ(I)>	40.2
Completeness [%]	99.4
Redundancy	11.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M sodium cacodylate:HCl pH 6.5, 1.26 M ammonium sulfate