5XNS
Crystal structure of the Smc head domain with an extended coiled coil bound to the C-terminal domain of ScpA derived from Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-31 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.304, 150.003, 51.081 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.932 - 2.010 |
R-factor | 0.2082 |
Rwork | 0.207 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4i99 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.957 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MR-Rosetta |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.010 |
Number of reflections | 45242 |
<I/σ(I)> | 31.6 |
Completeness [%] | 92.8 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 1M Ammonium citrate dibasic, 0.1M Sodium acetate trihydrate pH 4.6, 0.3M NDSB-195 |