5XNQ
Crystal structures of human SALM5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 152.601, 152.601, 93.822 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.875 - 2.802 |
| R-factor | 0.2489 |
| Rwork | 0.248 |
| R-free | 0.26890 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.805 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.056 | 0.588 |
| Number of reflections | 16115 | 786 |
| <I/σ(I)> | 36.875 | 3.4 |
| Completeness [%] | 98.8 | 99.2 |
| Redundancy | 6.4 | 6.6 |
| CC(1/2) | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2% PEG 3000, 0.1 M sodium acetate, 0.2 M lithium sulfate |
