5XNP
Crystal structures of human SALM5 in complex with human PTPdelta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 249.384, 249.384, 249.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.908 - 3.729 |
| R-factor | 0.2332 |
| Rwork | 0.232 |
| R-free | 0.26380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xnq 4yh7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.739 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.790 |
| High resolution limit [Å] | 3.729 | 3.729 |
| Rmerge | 0.109 | 0.919 |
| Number of reflections | 26924 | 1340 |
| <I/σ(I)> | 18.75 | 2.5 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.1 | 7.2 |
| CC(1/2) | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Tartrate Na/K, MES pH 6.0, lithium sulfate |
