5XNH
Crystal structure of the branched-chain polyamine synthase (BpsA) in complex with spermidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.869, 78.783, 79.407 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 50.010 - 1.950 |
| R-factor | 0.17391 |
| Rwork | 0.171 |
| R-free | 0.22493 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.804 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.010 |
| High resolution limit [Å] | 1.950 |
| Number of reflections | 47518 |
| <I/σ(I)> | 12.4 |
| Completeness [%] | 99.9 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Sodium chloride, 0.1M Tris pH 8.5, 12 % w/v PEG 4000 |
