5XN9
Crystal structure of a secretary abundant heat soluble (SAHS) protein from Ramazzottius varieornatus (from monomer sample)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-16 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.90000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.866, 57.505, 111.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.259 - 1.449 |
R-factor | 0.1457 |
Rwork | 0.145 |
R-free | 0.17190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.763 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.480 |
High resolution limit [Å] | 1.449 | 1.449 |
Rmeas | 0.077 | 0.933 |
Number of reflections | 62308 | 3067 |
<I/σ(I)> | 42.3 | 1.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 11.8 | 7.3 |
CC(1/2) | 0.786 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 12 mg/mL protein, 100 mM HEPES pH 7.8, 150 mM MgCl2, 1 mM ZnSO4, 3.0% v/v 2-propanol, and 21% v/v PEG 600 |