5XMA
Crystal structure of AsfvPolX in complex with DNA enzyme at P43212 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.846, 78.846, 241.677 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.353 - 3.800 |
R-factor | 0.2596 |
Rwork | 0.258 |
R-free | 0.28540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hrb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.581 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.940 |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 7857 | 734 |
<I/σ(I)> | 12.2 | 1.5 |
Completeness [%] | 96.8 | 92.9 |
Redundancy | 8.7 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Tris pH 7.0, 15% v/v ethanol |