5XM8
Crystal structure of AsfvPolX in complex with DNA enzyme and Pb.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.727, 118.848, 235.979 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.712 - 2.550 |
R-factor | 0.238 |
Rwork | 0.235 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hrb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.916 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.060 | 0.430 |
Number of reflections | 45470 | 4339 |
<I/σ(I)> | 26.8 | 2.3 |
Completeness [%] | 98.6 | 95.5 |
Redundancy | 7.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M di-ammonium hydrogen phosphate, 20% PEG 3350 |