5XM5
Crystal structure of Zinc binding protein ZinT at 1.49 Angstrom from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.021, 65.724, 73.532 |
Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
Resolution | 31.228 - 1.493 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aw8 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.249 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.228 | 1.520 |
High resolution limit [Å] | 1.493 | 1.490 |
Number of reflections | 56913 | 54181 |
<I/σ(I)> | 13.319 | 16.37 |
Completeness [%] | 90.5 | 90.5 |
Redundancy | 2.2 | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 288 | 22.5%(w/v) polyethylene glycol 4000, 0.135M ammonium acetate, and 0.1M sodium acetate trihydrate (pH 4.6) |