5XM5
Crystal structure of Zinc binding protein ZinT at 1.49 Angstrom from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.021, 65.724, 73.532 |
| Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
| Resolution | 31.228 - 1.493 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.23380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aw8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.228 | 1.520 |
| High resolution limit [Å] | 1.493 | 1.490 |
| Number of reflections | 56913 | 54181 |
| <I/σ(I)> | 13.319 | 16.37 |
| Completeness [%] | 90.5 | 90.5 |
| Redundancy | 2.2 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 288 | 22.5%(w/v) polyethylene glycol 4000, 0.135M ammonium acetate, and 0.1M sodium acetate trihydrate (pH 4.6) |
