5XM2

Human N-terminal domain of FACT complex subunit SPT16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	F 2 3
Unit cell lengths	247.983, 247.983, 247.983
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.330 - 2.187
R-factor	0.1687
Rwork	0.166
R-free	0.21650
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.028
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.187	2.190
Rmerge	0.150	0.860
Rpim	0.035	0.194
Number of reflections	64924	4271
<I/σ(I)>	22.6	2.8
Completeness [%]	100.0	100
Redundancy	20.5	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	Buffer composition of protein solution:20mM Hepes pH7.0, 200mM NaCl,1mM DTT,1Mm EDTA,5%Glycerol; Composition of reservoir protein: 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 23% v/v Jeffamine ED-2001 pH 7.0; Protein concentration 19.7 (mg /ml);