5XM0

The mouse nucleosome structure containing H2A, H2B type3-A, H3.3, and H4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	106.523, 110.095, 182.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.904 - 2.874
R-factor	0.2208
Rwork	0.219
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av2
RMSD bond length	0.010
RMSD bond angle	1.194
Data scaling software	HKL-2000 (704w)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.970
High resolution limit [Å]	2.870	6.180	2.870
Rmerge	0.082	0.037	0.500
Rmeas	0.094	0.043	0.574
Rpim	0.045	0.021	0.278
Total number of observations	203874
Number of reflections	49349
<I/σ(I)>	11.6
Completeness [%]	99.6	97.6	100
Redundancy	4.1	3.9	4.2
CC(1/2)		0.998	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	potassium cacodylate, potassium chloride, manganese chloride