5XLU
High Resolution Crystal Structure of Bacillus Licheniformis Gamma Glutamyl Transpeptidase with Acivicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-06-01 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.005, 85.732, 124.664 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.640 - 1.450 |
R-factor | 0.17508 |
Rwork | 0.174 |
R-free | 0.20513 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ott |
RMSD bond length | 0.023 |
RMSD bond angle | 2.171 |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.640 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 92246 | |
<I/σ(I)> | 34.01 | |
Completeness [%] | 96.1 | |
Redundancy | 9.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG MME 2000, 200mM magnesium chloride, 100mM Tris-Cl, 5mM acivicin VAPOR DIFFUSION, HANGING DROP, temperature 277K |