5XLU
High Resolution Crystal Structure of Bacillus Licheniformis Gamma Glutamyl Transpeptidase with Acivicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.005, 85.732, 124.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.640 - 1.450 |
| R-factor | 0.17508 |
| Rwork | 0.174 |
| R-free | 0.20513 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ott |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.171 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.640 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 92246 | |
| <I/σ(I)> | 34.01 | |
| Completeness [%] | 96.1 | |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG MME 2000, 200mM magnesium chloride, 100mM Tris-Cl, 5mM acivicin VAPOR DIFFUSION, HANGING DROP, temperature 277K |
