5XLJ

Crystal structure of the flagellar cap protein flid D2-D3 domains from serratia marcescens in Space group P432

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-18
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00004
Spacegroup name	P 4 3 2
Unit cell lengths	120.386, 120.386, 120.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
R-factor	0.19034
Rwork	0.189
R-free	0.20841
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5h5w
RMSD bond length	0.014
RMSD bond angle	1.463
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.113	0.517
Number of reflections	24119
<I/σ(I)>	33
Completeness [%]	99.9
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	6% PEG 6000, 2.0M sodium chloride