5XLA
The structure of hemagglutinin G228S mutant from an avian-origin H4N6 influenza virus in complex with human receptor analog LSTc
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.722, 100.722, 686.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.266 - 2.100 |
R-factor | 0.2118 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ha0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.063 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.791 | |
Number of reflections | 79407 | |
<I/σ(I)> | 20.7 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.4 | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M Bis-Tris (pH 6.5) and 20% (w/v) PEG 1,500 |