5XL2
The structure of hemagglutininfrom a swine-origin H4N6 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-01-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.419, 120.559, 132.278 |
Unit cell angles | 90.00, 92.29, 90.00 |
Refinement procedure
Resolution | 35.570 - 2.300 |
R-factor | 0.1857 |
Rwork | 0.184 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ha0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.039 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.331 | |
Number of reflections | 91122 | |
<I/σ(I)> | 17.4 | 5.8 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 5.8 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 2% (v/v) Tacsimate (pH 8.0), 0.1 M Tris (pH 8.5), and 16% (w/v) PEG 3,350 |