5XJX
Pre-formed plant receptor ERL1-TMM complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-10-07 |
Detector | MAC Science DIP-320 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 116.172, 114.395, 191.952 |
Unit cell angles | 89.57, 90.46, 59.86 |
Refinement procedure
Resolution | 43.249 - 3.055 |
R-factor | 0.2547 |
Rwork | 0.254 |
R-free | 0.27610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mn8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.702 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.090 |
High resolution limit [Å] | 3.050 | 3.055 |
Number of reflections | 158048 | |
<I/σ(I)> | 14 | |
Completeness [%] | 98.5 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 4% v/v Tacsimate pH 6.0, 10% w/v Polyethylene glycol 3350 |