5XJQ

Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1(1-82)/D2/F/E/G from Human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	83.320, 115.760, 128.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 3.280
R-factor	0.2013
Rwork	0.198
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3S6N
RMSD bond length	0.011
RMSD bond angle	1.540
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	60.000
High resolution limit [Å]	3.280
Number of reflections	19335
<I/σ(I)>	13.6
Completeness [%]	99.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	6% PEG8000, 100mM Tris.HCl