5XJD
TEAD in complex with fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.500, 74.500, 252.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.220 |
| R-factor | 0.19937 |
| Rwork | 0.198 |
| R-free | 0.22342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l15 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.524 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 36473 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 99.9 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | 2.6M sodium formate, 0.1M Tris pH 8.4, 5 % glycerol, 2mM magnesium chloride |
