5XJ9
Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the orthophosphate form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 58.339, 97.986, 83.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.730 - 1.830 |
R-factor | 0.2099 |
Rwork | 0.205 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.860 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.730 | 1.890 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.175 | 0.889 |
Rpim | 0.075 | 0.433 |
Number of reflections | 21613 | |
<I/σ(I)> | 7.02 | 2.28 |
Completeness [%] | 95.3 | 86.02 |
Redundancy | 6 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 277 | 1 mol% palmitoyl lysophosphatidic acid, 0.4 M Na2HPO4, 24% PEG 400, 0.1M HEPES pH 7.0 |