5XJ7
Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the acyl phosphate form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97854 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.224, 65.599, 84.678 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.573 - 1.772 |
R-factor | 0.1969 |
Rwork | 0.195 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.973 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.220 | 1.810 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.064 | 0.798 |
Rpim | 0.029 | 0.429 |
Number of reflections | 25689 | |
<I/σ(I)> | 18 | 2 |
Completeness [%] | 98.4 | 97.1 |
Redundancy | 5.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8 | 277 | 7.8 monoacylglycerol (7.8 MAG), 0.6M potassium fluoride, 22-26% PEG 400, 0.1M Tris HCl pH 8.0 |