5XHX
Crystal structure of VqsR LBD domain from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9791 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 52.834, 135.198, 150.772 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.414 - 2.100 |
R-factor | 0.2049 |
Rwork | 0.203 |
R-free | 0.23950 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.888 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 32086 |
<I/σ(I)> | 26.04 |
Completeness [%] | 99.9 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.01M Magnesium acetate tetrahydrate 0.05M Sodium cacodylate trihydrate pH 6.5 1.3M Lithium sulfate monohydrate |