5XHV

Crystal Structure Of Fab S40 In Complex With Influenza Hemagglutinin, HA1 subunit.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	94
Detector technology	CCD
Collection date	2016-04-17
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	C 1 2 1
Unit cell lengths	200.395, 133.640, 133.136
Unit cell angles	90.00, 110.47, 90.00

Refinement procedure

Resolution	25.000 - 3.350
R-factor	0.23227
Rwork	0.230
R-free	0.27452
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w9d 3hc4 3gbn
RMSD bond length	0.010
RMSD bond angle	1.494
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.470
High resolution limit [Å]	3.350	3.350
Rmerge	0.096	0.645
Number of reflections	34636
<I/σ(I)>	17.3	2.4
Completeness [%]	99.6	100
Redundancy	4.6	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	8%(w/v) PEG 6000, 1N NaOH, 15% MPD, 0.1 M Na HEPES pH 7.5