5XHP
Transferase with ligands
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 |
Unit cell lengths | 50.116, 50.116, 201.704 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.402 - 2.800 |
R-factor | 0.28994 |
Rwork | 0.288 |
R-free | 0.31864 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.527 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.800 |
Number of reflections | 13266 |
<I/σ(I)> | 2.6 |
Completeness [%] | 99.8 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 290 | 0.2M ammonium acetate, 0.1M Bis-Tris[pH5.5], 25% PEG2250 |