5XHN
Crystal structure of Frog M-ferritin K104E mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2016-01-11 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 184.210, 184.210, 184.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.270 - 1.630 |
R-factor | 0.16446 |
Rwork | 0.163 |
R-free | 0.19158 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ka3 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.283 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.270 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 33970 | |
<I/σ(I)> | 33.8 | 5.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.4 | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2.0 M MgCl2 and 100 mM Bicine (pH 9.0) |