5XHB

Crystal structure of the full length of NisI in a lipid free form, the nisin immunity protein, from Lactococcus lactis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-03
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97917
Spacegroup name	P 21 21 21
Unit cell lengths	45.984, 76.866, 76.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.444 - 1.900
R-factor	0.1789
Rwork	0.176
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.021
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.900
Number of reflections	21483
<I/σ(I)>	11.1
Completeness [%]	98.3
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	30-40mM ammonium sulfate, 0.1M Na-acetate pH 4.6, 16-18% PEG4000