5XH8
Aspergillus kawachii beta-fructofuranosidase complexed with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.799, 108.799, 152.043 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.110 - 2.100 |
R-factor | 0.17253 |
Rwork | 0.171 |
R-free | 0.20245 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ldk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.426 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.110 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 | 0.480 |
Number of reflections | 61305 | |
<I/σ(I)> | 53.6 | 7.9 |
Completeness [%] | 99.9 | |
Redundancy | 11.1 | 11.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate, 2.6 M sodium formate |