5XGX
Crystal structure of colwellia psychrerythraea strain 34H isoaspartyl dipeptidase E80Q mutant complexed with beta-isoaspartyl lysine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 107.878, 107.878, 155.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.330 |
R-factor | 0.19177 |
Rwork | 0.189 |
R-free | 0.24636 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.840 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.010 |
High resolution limit [Å] | 2.330 |
Number of reflections | 36407 |
<I/σ(I)> | 31.8 |
Completeness [%] | 97.1 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis-Tris propane pH 7.0 1 M ammonium citrate tribasic pH 7.0 |