5XGG
Crystal Structure C-terminal SH3 domain of Myosin IB from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.110, 77.670, 61.830 |
| Unit cell angles | 90.00, 108.24, 90.00 |
Refinement procedure
| Resolution | 58.790 - 1.720 |
| R-factor | 0.18627 |
| Rwork | 0.185 |
| R-free | 0.21878 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iim |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.969 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.670 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.110 | 0.430 |
| Rpim | 0.027 | 0.185 |
| Number of reflections | 101866 | |
| <I/σ(I)> | 4.17 | 4.17 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 6.5 | 6.2 |
| CC(1/2) | 0.997 | 0.960 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.2M ammonium sulphate, 5% v/v isopropanol |
