5XG3
Crystal structure of the ATPgS-engaged Smc head domain with an extended coiled coil bound to the C-terminal domain of ScpA derived from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.103, 104.783, 185.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.989 - 3.500 |
R-factor | 0.2631 |
Rwork | 0.261 |
R-free | 0.29560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xex |
RMSD bond length | 0.010 |
RMSD bond angle | 1.312 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 35074 |
<I/σ(I)> | 12.3 |
Completeness [%] | 92.6 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 10% PEG 3000, 0.1M Imidazole pH8.0, 0.2M LiSO4, 0.05M Hexamine cobalt (III) chloride |