5XG2
Crystal structure of a coiled-coil segment (residues 345-468 and 694-814) of Pyrococcus yayanosii Smc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.175, 53.710, 71.399 |
| Unit cell angles | 90.00, 90.83, 90.00 |
Refinement procedure
| Resolution | 36.171 - 2.000 |
| R-factor | 0.218 |
| Rwork | 0.213 |
| R-free | 0.25920 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.280 | |
| Rpim | 0.025 | |
| Number of reflections | 17619 | 725 |
| <I/σ(I)> | 23.8 | 2.06 |
| Completeness [%] | 94.4 | 78.5 |
| Redundancy | 4.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 0.18M HEPES (pH 7.0), 49% (+/-)-2-Methyl-2,4-pentanediol |
