5XFR

Ternary complex of MTF2, DNA and histone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2016-10-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 31 2 1
Unit cell lengths	137.743, 137.743, 101.226
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.593 - 2.250
R-factor	0.2043
Rwork	0.203
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xfn
RMSD bond length	0.003
RMSD bond angle	0.634
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.250	2.250
Rmerge	0.124	0.894
Number of reflections	52840
<I/σ(I)>	19.6	3
Completeness [%]	100.0	100
Redundancy	16.9	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M MES monohydrate-pH 6.5, 0.2M ammonium sulfate, 25% PEG monomethyl ether 5000, 10% glycerol