5XFJ

Crystal structure of LY2874455 in complex of FGFR4 gatekeeper mutation (V550M)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.54178
Spacegroup name	P 43 21 2
Unit cell lengths	62.216, 62.216, 184.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.727 - 3.250
R-factor	0.2845
Rwork	0.279
R-free	0.33630
RMSD bond length	0.005
RMSD bond angle	0.967
Data reduction software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.730
High resolution limit [Å]	3.250
Number of reflections	6209
<I/σ(I)>	19.18
Completeness [%]	99.3
Redundancy	22.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.65M NH2H2PO4