5XF1
Structure of the Full-length glucagon class B G protein-coupled receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.460, 248.790, 93.330 |
Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
Resolution | 46.920 - 3.190 |
R-factor | 0.2066 |
Rwork | 0.205 |
R-free | 0.23148 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l6r |
RMSD bond length | 0.011 |
RMSD bond angle | 1.397 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.190 |
Number of reflections | 49677 |
<I/σ(I)> | 3.1 |
Completeness [%] | 92.8 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100mM HEPES, pH7.0, 200mM potassium phospphate monobasic, 20% PEG500DME, 10 mM gly-gly-glysine |