5XF1
Structure of the Full-length glucagon class B G protein-coupled receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.460, 248.790, 93.330 |
| Unit cell angles | 90.00, 90.16, 90.00 |
Refinement procedure
| Resolution | 46.920 - 3.190 |
| R-factor | 0.2066 |
| Rwork | 0.205 |
| R-free | 0.23148 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l6r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.190 |
| Number of reflections | 49677 |
| <I/σ(I)> | 3.1 |
| Completeness [%] | 92.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100mM HEPES, pH7.0, 200mM potassium phospphate monobasic, 20% PEG500DME, 10 mM gly-gly-glysine |
