5XEZ
Structure of the Full-length glucagon class B G protein-coupled receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2016-08-20 |
Detector | CS-PAD CXI-1 |
Wavelength(s) | 1.3 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.610, 245.330, 96.150 |
Unit cell angles | 90.00, 90.01, 90.00 |
Refinement procedure
Resolution | 31.780 - 3.000 |
R-factor | 0.21123 |
Rwork | 0.210 |
R-free | 0.24327 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4l6r |
RMSD bond length | 0.008 |
RMSD bond angle | 1.414 |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 67598 |
<I/σ(I)> | 4.8 |
Completeness [%] | 100.0 |
Redundancy | 198 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | 100mM HEPES, pH7.0, 300mM potassium phosphate monobasic, 25% PEG500DME, 100mM gly-gly-glycine |