5XEI
Crystal structure of the Smc head domain with a coiled coil and joint derived from Pyrococcus yayanosii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97941 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.320, 50.836, 122.057 |
Unit cell angles | 90.00, 118.09, 90.00 |
Refinement procedure
Resolution | 39.625 - 2.599 |
R-factor | 0.2145 |
Rwork | 0.207 |
R-free | 0.27840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xns |
RMSD bond length | 0.010 |
RMSD bond angle | 1.181 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.599 |
Number of reflections | 18871 |
<I/σ(I)> | 13.7 |
Completeness [%] | 92.8 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 7% PEG 3350, 4% Tacsimate pH4.0 |