5XEG
The structure of OsALKBH1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-01-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 50.150, 67.729, 67.967 |
| Unit cell angles | 90.00, 99.89, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.16351 |
| Rwork | 0.161 |
| R-free | 0.20778 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3khb |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.170 |
| Number of reflections | 20894 | |
| <I/σ(I)> | 32.6 | 6.6 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | 1.8 |
| CC(1/2) | 0.965 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | MPD(2-methyl-1,3-propanediol), HEPES Sodium |
